Difference between revisions of "Part:BBa K5382151:Design"
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===Source=== | ===Source=== | ||
| − | The z domain is derived from the B domain of protein A. Protein A is a protein widely found on the cell wall of Staphylococcus aureus. The extracellular portion of protein A contains five highly homologous IgG binding domains, named E, D, A, B, and C from the N-terminal, each containing approximately 58 amino acid residues. The z domain is an engineered analogue of the B domain, originally developed as an affinity purification treatment for fusion protein production. In contrast to the B domain, the z domain contains two amino acid substitutes (Ala1→Val and Gly29→Ala). | + | The z domain is derived from the B domain of protein A. Protein A is a protein widely found on the cell wall of <i>Staphylococcus aureus</i>. The extracellular portion of protein A contains five highly homologous IgG binding domains, named E, D, A, B, and C from the N-terminal, each containing approximately 58 amino acid residues. The z domain is an engineered analogue of the B domain, originally developed as an affinity purification treatment for fusion protein production. In contrast to the B domain, the z domain contains two amino acid substitutes (Ala1→Val and Gly29→Ala).<br> |
4z domain is an engineered tetramer of z domain, which is formed by four z domains in series through GS linker, and the genes encoding them are expressed in series. | 4z domain is an engineered tetramer of z domain, which is formed by four z domains in series through GS linker, and the genes encoding them are expressed in series. | ||
Latest revision as of 12:31, 2 October 2024
4z domain-protein domain
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
Source
The z domain is derived from the B domain of protein A. Protein A is a protein widely found on the cell wall of Staphylococcus aureus. The extracellular portion of protein A contains five highly homologous IgG binding domains, named E, D, A, B, and C from the N-terminal, each containing approximately 58 amino acid residues. The z domain is an engineered analogue of the B domain, originally developed as an affinity purification treatment for fusion protein production. In contrast to the B domain, the z domain contains two amino acid substitutes (Ala1→Val and Gly29→Ala).
4z domain is an engineered tetramer of z domain, which is formed by four z domains in series through GS linker, and the genes encoding them are expressed in series.