Difference between revisions of "Part:BBa K5127021:Design"
 
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===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E. coli</i>.
  
  

Latest revision as of 12:24, 2 October 2024


J23106-BreR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1012
    Illegal XbaI site found at 775
    Illegal SpeI site found at 750
    Illegal PstI site found at 345
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1012
    Illegal NheI site found at 781
    Illegal NheI site found at 804
    Illegal SpeI site found at 750
    Illegal PstI site found at 345
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1012
    Illegal BamHI site found at 1018
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1012
    Illegal XbaI site found at 775
    Illegal SpeI site found at 750
    Illegal PstI site found at 345
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1012
    Illegal XbaI site found at 775
    Illegal SpeI site found at 750
    Illegal PstI site found at 345
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.


Source

N/A


References

N/A