Difference between revisions of "Part:BBa K5322037"

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__NOTOC__
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<partinfo>BBa_K5322037 short</partinfo>
  
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__TOC__
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==Usage and Biology==
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<p>
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In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed  plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments.
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</p>
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<style>
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  .center-img {
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    text-align:center;
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<div class="center-img">
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<img src="https://static.igem.wiki/teams/5322/wet-lab/101-p-soxr-t-psoxs-rbs-egfp-map.png" alt="pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7" width="300">
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<p align="center"><b>Figure 1-1</b>  Plasmid pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7</p>
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</div>
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</html>
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==Sequence and Features==
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<partinfo>BBa_K5322037 SequenceAndFeatures</partinfo>

Latest revision as of 12:18, 2 October 2024

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7

Usage and Biology

In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments.

pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7

Figure 1-1 Plasmid pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 683
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 702
    Illegal NheI site found at 725
    Illegal NheI site found at 1475
    Illegal SpeI site found at 683
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 683
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 683
  • 1000
    COMPATIBLE WITH RFC[1000]