Difference between revisions of "Part:BBa K5236015"

 
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RBS: the location to bind ribosome.  
 
RBS: the location to bind ribosome.  
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IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.
 
IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.
  
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<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95f-sequnce-cycle-3.png" width = "50%"><br></html></center>
 
<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95f-sequnce-cycle-3.png" width = "50%"><br></html></center>
<center>Fig.1 The result of DNA sequencing  </center>
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<center>Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing  </center>
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<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95s-plasmid.png" width = "50%"><br></html></center>
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<center>Fig.2 Constructed </center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:12, 2 October 2024


pET28a-pelB-IsPETase(K95S)-6xHis

This composite part codes for the mutated IsPETase K95S and expresses the enzyme in the extracellular space of chassis.

T7 promoter:used to regulate gene expression of recombinantproteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.


Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing



Fig.2 Constructed


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005