Difference between revisions of "Part:BBa K5236015"
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<partinfo>BBa_K5236015 short</partinfo> | <partinfo>BBa_K5236015 short</partinfo> | ||
| − | T7 promoter: | + | This composite part codes for the mutated IsPETase K95S and expresses the enzyme in the extracellular space of chassis. |
| − | used to | + | |
| − | regulate gene | + | T7 promoter:used to regulate gene expression of recombinantproteins. |
| − | expression of | + | |
| − | recombinantproteins. | + | Lac operator: used to control gene expression by lactose. |
| − | Lac operator: | + | |
| − | used to control | + | RBS: the location to bind ribosome. |
| − | gene | + | |
| − | expression by | + | IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli. |
| − | lactose. | + | |
| − | RBS: the | + | pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. |
| − | location to | + | |
| − | bind ribosome. | + | 6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. |
| − | IsPETase K95S: | + | |
| − | This sequence | + | T7 terminator: terminates transcription. |
| − | encodes for a | + | |
| − | mutated | + | <center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95f-sequnce-cycle-3.png" width = "50%"><br></html></center> |
| − | IsPETase K95S | + | <center>Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing </center> |
| − | and is derived | + | |
| − | from | + | |
| − | Escherichia coli. | + | <center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95s-plasmid.png" width = "50%"><br></html></center> |
| − | pelB: the | + | <center>Fig.2 Constructed </center> |
| − | sequence | + | |
| − | codes for | + | |
| − | signal peptide | + | |
| − | which helps | + | |
| − | PETase exit the | + | |
| − | cell membrane. | + | |
| − | 6xHis: a tag for | + | |
| − | the purification | + | |
| − | of protein | + | |
| − | which focus in | + | |
| − | the production | + | |
| − | of recombinant | + | |
| − | proteins. | + | |
| − | T7 terminator: | + | |
| − | terminates | + | |
| − | transcription. | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 12:12, 2 October 2024
pET28a-pelB-IsPETase(K95S)-6xHis
This composite part codes for the mutated IsPETase K95S and expresses the enzyme in the extracellular space of chassis.
T7 promoter:used to regulate gene expression of recombinantproteins.
Lac operator: used to control gene expression by lactose.
RBS: the location to bind ribosome.
IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.
pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.
6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.
T7 terminator: terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1005