Difference between revisions of "Part:BBa K220000:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | |||
+ | '''!!! WARNING: Pst1 cut site within gene !!!''' | ||
+ | |||
'''Length:''' 56 | '''Length:''' 56 | ||
'''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3' | '''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3' |
Latest revision as of 14:17, 2 November 2009
Sarcoisine Dimethyl Glycine Methyl Transferase (SDMT)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 777
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 777
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 71
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 777
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 777
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 836
Design Notes
!!! WARNING: Pst1 cut site within gene !!!
Length: 56 Fwd: 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
Length: 33 Rev: 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'
Source
Gene isolation from Galdieria sulphuraria
References
J McCoy, L Bailey, Y NG, C Bingman, R Wrobel, A Weber, B Fox, G Philips Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria