Difference between revisions of "Part:BBa K220000:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K220000 short</partinfo>
 
<partinfo>BBa_K220000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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 +
'''!!! WARNING: Pst1 cut site within gene !!!'''
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'''Length:''' 56  
 
'''Length:''' 56  
 
'''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
 
'''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
  
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'''Length:'' 33   
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'''Length:''' 33   
 
'''Rev:''' 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'
 
'''Rev:''' 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'
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Gene isolation from Galdieria sulphuraria
 
Gene isolation from Galdieria sulphuraria
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===References===
 
===References===
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 +
J McCoy, L Bailey, Y NG, C Bingman, R Wrobel, A Weber, B Fox, G Philips
 +
''Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria''

Latest revision as of 14:17, 2 November 2009

Sarcoisine Dimethyl Glycine Methyl Transferase (SDMT)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 777
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


Design Notes

!!! WARNING: Pst1 cut site within gene !!!

Length: 56 Fwd: 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'

Length: 33 Rev: 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'


Source

Gene isolation from Galdieria sulphuraria


References

J McCoy, L Bailey, Y NG, C Bingman, R Wrobel, A Weber, B Fox, G Philips Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria