Difference between revisions of "Part:BBa K5443010"

 
 
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The <b>COMT enzyme (Caffeic acid O-methyltransferase)</b>, from <i>Synechocystis PCC6714</i>, functions as the third step in our vanillin biosynthesis pathway. This enzyme catalyses the methylation of caffeic acid by transferring a methyl group to the hydroxyl group of the caffeic acid molecule, converting it into ferulic acid. This methylation step is crucial for forming the characteristic methoxy group of vanillin.
  
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__NOTOC__
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This part is new to the Registry, but is homologous to earlier COMT parts contributed by earlier iGEM teams: [https://parts.igem.org/Part:BBa_I742107 BBa_I742107] and [https://parts.igem.org/Part:BBa_K1129041 BBa_K1129041].
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<partinfo>BBa_K5443010 short</partinfo>
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These enzymes methylate caffeic acid to produce ferulic acid, a direct precursor to vanillin. The methylation step is crucial for forming the characteristic methoxy group of vanillin.
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/comt-reaction.jpg" style="width:600px;height:auto">
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<!-- Add more about the biology of this part here
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    <figcaption>
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===Usage and Biology===
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        <b>Figure 1. COMT-catalysed conversion of caffeic acid to ferulic acid.</b><br>
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        <i>This reaction represents the third step in our vanillin synthesis pathway. The COMT enzyme converts caffeic acid into ferulic acid. Note that this is actually a two-step reaction performed by the same enzyme; the intermediate reaction has been omitted for simplicity.</i>
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    </figcaption>
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<span class='h3bb'>Sequence and Features</span>
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</figure>
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<partinfo>BBa_K5443010 SequenceAndFeatures</partinfo>
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</html>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K5443010 parameters</partinfo>
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Latest revision as of 11:44, 2 October 2024

The COMT enzyme (Caffeic acid O-methyltransferase), from Synechocystis PCC6714, functions as the third step in our vanillin biosynthesis pathway. This enzyme catalyses the methylation of caffeic acid by transferring a methyl group to the hydroxyl group of the caffeic acid molecule, converting it into ferulic acid. This methylation step is crucial for forming the characteristic methoxy group of vanillin.

This part is new to the Registry, but is homologous to earlier COMT parts contributed by earlier iGEM teams: BBa_I742107 and BBa_K1129041.

Figure 1. COMT-catalysed conversion of caffeic acid to ferulic acid.
This reaction represents the third step in our vanillin synthesis pathway. The COMT enzyme converts caffeic acid into ferulic acid. Note that this is actually a two-step reaction performed by the same enzyme; the intermediate reaction has been omitted for simplicity.