Difference between revisions of "Part:BBa K5443001:Experience"
 
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We succeeded in producing at least a little bit of vanillin from tyrosine, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 at least were all functional - see LC-MS data below.  
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Our LC-MS analysis showed the presence of p-coumarate (0.3 ppm) in reactions, and we also succeeded in producing a small amount of vanillin (0.2 ppm), and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including TAL-Sam8. See LC-MS data below (Fig. 1).
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    <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
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        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11.</i>
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https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
 
  
 
===Applications of BBa_K5443001===
 
===Applications of BBa_K5443001===

Latest revision as of 11:30, 2 October 2024

Our LC-MS analysis showed the presence of p-coumarate (0.3 ppm) in reactions, and we also succeeded in producing a small amount of vanillin (0.2 ppm), and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including TAL-Sam8. See LC-MS data below (Fig. 1).

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11.


Applications of BBa_K5443001

User Reviews

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