Difference between revisions of "Part:BBa K5198104"

Latest revision as of 10:50, 2 October 2024

Igk-NanoLuc
NanoLuciferase (BBa_K1734004 ) with Ig Kappa tag (BBa_K4646003 ). This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS (Figure 1).

Figure 1. Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 503

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 __NOTOC__
 <partinfo>BBa_K5198104 short</partinfo>
+<html>
+<br>
+NanoLuciferase (<a href="https://parts.igem.org/Part:BBa_K1734004">BBa_K1734004</a>
+) with Ig Kappa tag (<a href="https://parts.igem.org/Part:BBa_K4646003">BBa_K4646003</a>
+). This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS (<b>Figure 1</b>).
-NanoLuciferase with Ig Kappa tag. This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
+<div style="text-align: center;">
+<img src="https://static.igem.wiki/teams/5198/results/figure-3v3.webp" alt="Description of the image" style="width: 400px; height: auto;">
+</div>
+<p><b>Figure 1.</b> Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.</p>
+</html>
 <!-- Add more about the biology of this part here