Difference between revisions of "Part:BBa K5127000:Design"
 
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===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E. coli</i>.
  
  
  
 
===Source===
 
===Source===
 +
Genome of <i>Vibrio fischeri</i>.
 +
  
−
N/A
 
  
 
===References===
 
===References===
−
sdahilkhclz
+
d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8
 +
 
 +
LaFleur, T. L., Hossain, A., & Salis, H. M. (2022). Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-32829-5
 +
 
 +
Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404
 +
 
 +
Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9, 3617. https://doi.org/10.1038/s41467-018-05857-3
 +
 
 +
Taketani, M., Zhang, J., Zhang, S., Triassi, A. J., Huang, Y. J., Griffith, L. G., & Voigt, C. A. (2020). Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron. Nature Biotechnology, 38(9), 962–969. https://doi.org/10.1038/s41587-020-0468-5

Latest revision as of 10:03, 2 October 2024


VFA0359


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250
    Illegal SapI.rc site found at 544


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.


Source

Genome of Vibrio fischeri.


References

d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8

LaFleur, T. L., Hossain, A., & Salis, H. M. (2022). Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-32829-5

Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404

Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9, 3617. https://doi.org/10.1038/s41467-018-05857-3

Taketani, M., Zhang, J., Zhang, S., Triassi, A. J., Huang, Y. J., Griffith, L. G., & Voigt, C. A. (2020). Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron. Nature Biotechnology, 38(9), 962–969. https://doi.org/10.1038/s41587-020-0468-5