Difference between revisions of "Part:BBa K5127016:Design"
 
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Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.
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<i>Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.</i>
 
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===Source===
 
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<i>E. Coli</i>
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E. Coli
 
  
 
===References===
 
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Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, & Sun Chang Kim. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Research, 36(14), e84–e84. https://doi.org/10.1093/nar/gkn359

Latest revision as of 10:02, 2 October 2024


Arabinose inducible Lambda Red system with reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1776
    Illegal AgeI site found at 3428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1758


Design Notes

The pRed-Aspink expresses the Lambda Red recombinase under the control of an arabinose-inducible promoter. The plasmid also constitutively expresses the chromoprotein, AsPink, which serves as a visual marker to confirm the presence of the plasmid in the host cells (Figure 1).


Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.


Source

E. Coli


References

Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, & Sun Chang Kim. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Research, 36(14), e84–e84. https://doi.org/10.1093/nar/gkn359