Difference between revisions of "Part:BBa K5115026"

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===Characterization===
 
===Characterization===
  
 
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 09:56, 2 October 2024


csoS2

contributed by Fudan iGEM 2024

Introduction

The csoS2 is a subunit of the csoS operon BBa_K5115034.

Usage and Biology

CsoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, CsoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, CsoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References