Difference between revisions of "Part:BBa K5291042"

Latest revision as of 09:49, 2 October 2024

CYPY96F

CYPY96F: P450cam mutant with higher alkane oxidation activity.

Usage and Biology

In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.

Fig.1 The map of plasmid pAB1-CYPY96F-VHb.

Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.

Fig.2 The SDS-PAGE result of CYPY96F.

Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 312
Illegal BamHI site found at 392
Illegal BamHI site found at 1221
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 109
Illegal NgoMIV site found at 277
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.<br>
-<html><img width = "700" src="https://static.igem.wiki/teams/5291/images/part-wyn/cyp-plasmid.png" /></html><br>
+<html><img width = "650" src="https://static.igem.wiki/teams/5291/images/part-wyn/cypvgb-map.png" /></html><br>
-<b>Fig.1 The map of plasmid pAB1-CYPY96F-VHB.</b><br><br>
+<b>Fig.1 The map of plasmid pAB1-CYPY96F-VHb.</b><br><br>
 Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.<br>
-<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-wyn/sds-page-cyp.png" /></html><br>
+<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-wyn/sds-vhb-white.png" /></html><br>
 <b>Fig.2 The SDS-PAGE result of CYPY96F.</b><br><br>
 Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.<br>