Difference between revisions of "Part:BBa K5291042"

 
 
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<partinfo>BBa_K5291042 short</partinfo>
 
<partinfo>BBa_K5291042 short</partinfo>
  
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CYPY96F is a protein-engineered variant of the cytochrome P450cam enzyme, designed to enhance its ability to catalyze the oxidation of alkanes. The Y96F mutation introduces phenylalanine at position 96, increasing the substrate-binding pocket's hydrophobicity. This modification is crucial for improving the enzyme's affinity for hydrophobic substrates like alkanes and reducing the enzyme's susceptibility to uncoupling, where water molecules interfere with the catalytic cycle. The CYPY96F variant shows significantly increased activity and product formation rates for alkane oxidation compared to the wild-type enzyme. Researchers and bioengineers can incorporate CYPY96F into projects that require selective oxidation of alkanes, such as in the synthesis of bioremediation, biofuels, or other specialty chemicals.
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CYPY96F: P450cam mutant with higher alkane oxidation activity.
  
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===Usage and Biology===
 
===Usage and Biology===
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In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.<br>
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<html><img width = "650" src="https://static.igem.wiki/teams/5291/images/part-wyn/cypvgb-map.png" /></html><br>
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<b>Fig.1 The map of plasmid pAB1-CYPY96F-VHb.</b><br><br>
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Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.<br>
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<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-wyn/sds-vhb-white.png" /></html><br>
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<b>Fig.2 The SDS-PAGE result of CYPY96F.</b><br><br>
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Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.<br>
  
 
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Latest revision as of 09:49, 2 October 2024


CYPY96F

CYPY96F: P450cam mutant with higher alkane oxidation activity.

Usage and Biology

In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.

Fig.1 The map of plasmid pAB1-CYPY96F-VHb.

Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.

Fig.2 The SDS-PAGE result of CYPY96F.

Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 312
    Illegal BamHI site found at 392
    Illegal BamHI site found at 1221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]