Difference between revisions of "Part:BBa K5291036"

 
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<partinfo>BBa_K5291036 short</partinfo>
 
<partinfo>BBa_K5291036 short</partinfo>
  
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This part is design to anchor the PEBP-GFP fusion protein to the membrane of bacteria. bacteria with PE-binding peptide (PEBP) are expected to have a stronger PE plastic binding capacity. And GFP is used to observe the bacteria on the PE surface to detect the binding ability of the engineering bacteria.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:29, 2 October 2024


pAB1-pS-PEBP-GFP

This part is design to anchor the PEBP-GFP fusion protein to the membrane of bacteria. bacteria with PE-binding peptide (PEBP) are expected to have a stronger PE plastic binding capacity. And GFP is used to observe the bacteria on the PE surface to detect the binding ability of the engineering bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1033
    Illegal BamHI site found at 996
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NgoMIV site found at 1351
    Illegal NgoMIV site found at 1720
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]