Difference between revisions of "Part:BBa K5242019"
| Line 3: | Line 3: | ||
<partinfo>BBa_K5242019 short</partinfo> | <partinfo>BBa_K5242019 short</partinfo> | ||
| − | + | The 2A peptides is a small, self-cleaving sequence derived from the foot-and-mouth disease virus (FMDV). The 2A peptide works by causing a ribosome to skip the final glycine of the 2A sequence, leading to a cleavage event that separates the proteins encoded upstream and downstream of the 2A sequence. We use E2A and LV2A to separate two fluorescent proteins and intermediate peptides. The GSG linker is used to link the 2A peptides with upstream peptides. | |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:11, 2 October 2024
E2A
The 2A peptides is a small, self-cleaving sequence derived from the foot-and-mouth disease virus (FMDV). The 2A peptide works by causing a ribosome to skip the final glycine of the 2A sequence, leading to a cleavage event that separates the proteins encoded upstream and downstream of the 2A sequence. We use E2A and LV2A to separate two fluorescent proteins and intermediate peptides. The GSG linker is used to link the 2A peptides with upstream peptides.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]