Difference between revisions of "Part:BBa K5335000:Design"

Latest revision as of 09:09, 2 October 2024

MS2 CP tandem dimer fusion containing SpyTag tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 331
Illegal BsaI site found at 760

Design Notes

No other details

Source

The series capsid protein sequence was artificially designed and synthesized. MS2 capsid subunits' sequence is from bacteriophage MS2.

References

[1] Peabody DS. Translational repression by bacteriophage MS2 coat protein expressed from a plasmid. A system for genetic analysis of a protein-RNA interaction. J Biol Chem. 1990 Apr 5;265(10):5684-9.
[2] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3.
[3] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3.
[4] 王丽.诺如病毒GⅡ.17型病毒样颗粒的纯化及免疫原性研究.2023.昆明医科大学,MA thesis.doi:10.27202/d.cnki.gkmyc.2023.001033.

@@ Line 16: / Line 16: @@
 ===References===
+[1] Peabody DS. Translational repression by bacteriophage MS2 coat protein expressed from a plasmid. A system for genetic analysis of a protein-RNA interaction. J Biol Chem. 1990 Apr 5;265(10):5684-9.<br>
+[2] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3.<br>
+[3] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3.<br>
+[4] 王丽.诺如病毒GⅡ.17型病毒样颗粒的纯化及免疫原性研究.2023.昆明医科大学,MA thesis.doi:10.27202/d.cnki.gkmyc.2023.001033.<br>