Difference between revisions of "Part:BBa K255004:Experience"
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BBa_K255004 represents a open loop system with constitutive expression of | BBa_K255004 represents a open loop system with constitutive expression of | ||
LacI and replication of plasmid copy. Both LacI and plasmid copy number | LacI and replication of plasmid copy. Both LacI and plasmid copy number | ||
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genome. Further the growth of the transformant was also characterized at | genome. Further the growth of the transformant was also characterized at | ||
different lactose concentrations. | different lactose concentrations. | ||
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Latest revision as of 16:24, 31 October 2009
BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein. YFP was measured using FACS and the distribution obtained was independent of IPTG. Beta-galactosidase was also measured at various lactose concentration to characterize the expression of gene in the host genome. Further the growth of the transformant was also characterized at different lactose concentrations.
Applications of BBa_K255004
This part was used as open loop system in our experiments.
User Reviews
This part works fine.
UNIQb8518248212d90d5-partinfo-00000000-QINU UNIQb8518248212d90d5-partinfo-00000001-QINU