Difference between revisions of "Part:BBa K255004:Experience"

Latest revision as of 16:24, 31 October 2009

BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein. YFP was measured using FACS and the distribution obtained was independent of IPTG. Beta-galactosidase was also measured at various lactose concentration to characterize the expression of gene in the host genome. Further the growth of the transformant was also characterized at different lactose concentrations.

Expression of BBa_K255004 in lacI deleted E.coli strain

Applications of BBa_K255004

This part was used as open loop system in our experiments.

User Reviews

This part works fine.

UNIQ3d48d72eb1cd2389-partinfo-00000000-QINU UNIQ3d48d72eb1cd2389-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>
 BBa_K255004 represents a open loop system with constitutive expression of
 LacI and replication of plasmid copy. Both LacI and plasmid copy number
@@ Line 10: / Line 9: @@
 genome. Further the growth of the transformant was also characterized at
 different lactose concentrations.
+[[Image:p4n.png|thumb|300px|right|alt=A |Expression of BBa_K255004 in lacI deleted E.coli strain]]
 ===Applications of BBa_K255004===
-This part was used as open loop system for our experiments.
+This part was used as open loop system in our experiments.
 ===User Reviews===
+This part works fine.
 <!-- DON'T DELETE --><partinfo>BBa_K255004 StartReviews</partinfo>
 <!-- Template for a user review