Difference between revisions of "Part:BBa K5335032"

 
 
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This is an aconite isomerase from Bacillus thuringiensis YBT-1520, which can catalyze the isomerization of cis-aconite to trans-aconite and its reverse reaction at the same time, but has a higher activity to catalyze the isomerization of cis-aconite to trans-aconite.
 
This is an aconite isomerase from Bacillus thuringiensis YBT-1520, which can catalyze the isomerization of cis-aconite to trans-aconite and its reverse reaction at the same time, but has a higher activity to catalyze the isomerization of cis-aconite to trans-aconite.
  
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== Sequence and Features ==
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5335032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5335032 SequenceAndFeatures</partinfo>
  
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== Usage and Biology ==
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TAA biosysnthesis-related gene <i>A</i> (<i>tbrA</i>) encodes a aconite isomerase in <i>Bacillus thuringiensis</i> YBT-1520, which can isomerize cis-aconite into rice aconite, and also has the ability to catalyze its reverse reaction. This property allows bacteria with such enzymes to isomerize TAA into CAA through aconite isomerase, thus growing on the medium with TAA as the only carbon source.[1]
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In our project, we aim to utilize the nematocidal activity of TAA as an alternative approach for killing nematodes. Additionally, we use the tbrAB genes, which encode an aconitate isomerase and a transport carrier, respectively. These genes allow the transport of TAA produced inside the bacteria to the extracellular environment, enabling our engineered bacteria to secrete TAA and thus exert a nematocidal effect.
  
 
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Latest revision as of 09:00, 2 October 2024


An aconite isomerase from Bacillus thuringiensis.

This is an aconite isomerase from Bacillus thuringiensis YBT-1520, which can catalyze the isomerization of cis-aconite to trans-aconite and its reverse reaction at the same time, but has a higher activity to catalyze the isomerization of cis-aconite to trans-aconite.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TAA biosysnthesis-related gene A (tbrA) encodes a aconite isomerase in Bacillus thuringiensis YBT-1520, which can isomerize cis-aconite into rice aconite, and also has the ability to catalyze its reverse reaction. This property allows bacteria with such enzymes to isomerize TAA into CAA through aconite isomerase, thus growing on the medium with TAA as the only carbon source.[1] In our project, we aim to utilize the nematocidal activity of TAA as an alternative approach for killing nematodes. Additionally, we use the tbrAB genes, which encode an aconitate isomerase and a transport carrier, respectively. These genes allow the transport of TAA produced inside the bacteria to the extracellular environment, enabling our engineered bacteria to secrete TAA and thus exert a nematocidal effect.