Difference between revisions of "Part:BBa K5443003"
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This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011 | This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011 | ||
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Revision as of 07:57, 2 October 2024
4-Hydroxyphenylacetate 3-hydroxylase (HpaBC) from E.coli W is an alternative enzyme to Sam5 to catalyse the hydroxylation of 4-coumaric acid to yield caffeic acid as the second step in the vanillin biosynthesis pathway. HpaBC is a two-component flavin adenine dinucleotide (FAD)-dependent monooxygenase that has a broad host range, and acts on a wide range of phenolic compounds. In our vanillin biosynthesis pathway, HpaBC is performing an ‘unnatural’ role in hydroxylating coumarate, since its physiological substrate is 4-hydroxyphenylacetate.
This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]