Difference between revisions of "Part:BBa K5443003"

 
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4-Hydroxyphenylacetate 3-hydroxylase (HpaBC) from E.coli W is an alternative enzyme to Sam5 to catalyse the hydroxylation of 4-coumaric acid to yield caffeic acid as the second step in the vanillin biosynthesis pathway. HpaBC is a two-component flavin adenine dinucleotide (FAD)-dependent monooxygenase that has a broad host range, and acts on a wide range of phenolic compounds. In our vanillin biosynthesis pathway, HpaBC is performing an ‘unnatural’ role in hydroxylating coumarate, since its physiological substrate is 4-hydroxyphenylacetate.
  
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This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011
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<partinfo>BBa_K5443003 short</partinfo>
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HpaBC converts 4-coumaric acid to caffeic acid, adding the caffeic hydroxyl group necessary for subsequent modifications in the pathway&#8203;&#8203;.
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Revision as of 07:56, 2 October 2024

4-Hydroxyphenylacetate 3-hydroxylase (HpaBC) from E.coli W is an alternative enzyme to Sam5 to catalyse the hydroxylation of 4-coumaric acid to yield caffeic acid as the second step in the vanillin biosynthesis pathway. HpaBC is a two-component flavin adenine dinucleotide (FAD)-dependent monooxygenase that has a broad host range, and acts on a wide range of phenolic compounds. In our vanillin biosynthesis pathway, HpaBC is performing an ‘unnatural’ role in hydroxylating coumarate, since its physiological substrate is 4-hydroxyphenylacetate.

This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]