|
|
| Line 7: |
Line 7: |
| | ===Design Notes=== | | ===Design Notes=== |
| | Biobrick Standard Prefix and BioFusion (aka SilverLab) suffix | | Biobrick Standard Prefix and BioFusion (aka SilverLab) suffix |
| − |
| |
| − | This part was designed as a plasmid vector backbone and was expected to work by adding an N-terminal Arginine Tag to the protein to which it would be fused.
| |
| − |
| |
| − | This part was shown to work via the sequencing information that we received.
| |
| − |
| |
| − | pSB-N-terminus
| |
| − | Score = 60.0 bits (30), Expect = 1e-15
| |
| − | Identities = 30/30 (100%), Gaps = 0/30 (0%)
| |
| − | Strand=Plus/Plus
| |
| − |
| |
| − | Query 10 (The vector sent in)
| |
| − | ATGCGCCGCCGCCGCCGCCGCCGCCGCCGC 39
| |
| − |
| |
| − | Sbjct 1 (the sequence expected)
| |
| − | ATGCGCCGCCGCCGCCGCCGCCGCCGCCGC 30
| |
| − |
| |
| − | We fused a YFP gene with an N-terminal (Biofusion standard prefix)
| |
| − |
| |
| − | mRBS-N-terminal EYFP
| |
| − | Score = 1379 bits (717), Expect = 0.0
| |
| − | Identities = 717/717 (100%), Gaps = 0/717 (0%)
| |
| − | Strand=Plus/Plus
| |
| − |
| |
| − | Query 147 (The vector sent in)
| |
| − | ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 206
| |
| − |
| |
| − | Sbjct 1 (the sequence expected)
| |
| − | ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 60
| |
| − |
| |
| − |
| |
| − | Query 207 GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTAC 266
| |
| − | Sbjct 61 GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTAC 120
| |
| − |
| |
| − |
| |
| − | Query 267 GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC 326
| |
| − | Sbjct 121 GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC 180
| |
| − |
| |
| − |
| |
| − | Query 327 CTCGTGACCACCTTCGGCTACGGCCTGCAATGCTTCGCCCGCTACCCCGACCACATGAAG 386
| |
| − | Sbjct 181 CTCGTGACCACCTTCGGCTACGGCCTGCAATGCTTCGCCCGCTACCCCGACCACATGAAG 240
| |
| − |
| |
| − |
| |
| − | Query 387 CTGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC 446
| |
| − | Sbjct 241 CTGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC 300
| |
| − |
| |
| − |
| |
| − | Query 447 TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTG 506
| |
| − | Sbjct 301 TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTG 360
| |
| − |
| |
| − |
| |
| − | Query 507 GTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC 566
| |
| − | Sbjct 361 GTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC 420
| |
| − |
| |
| − |
| |
| − | Query 567 AAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAAC 626
| |
| − | Sbjct 421 AAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAAC 480
| |
| − |
| |
| − |
| |
| − | Query 627 GGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCC 686
| |
| − | Sbjct 481 GGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCC 540
| |
| − |
| |
| − |
| |
| − | Query 687 GACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC 746
| |
| − | Sbjct 541 GACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC 600
| |
| − |
| |
| − |
| |
| − | Query 747 TACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTC 806
| |
| − | Sbjct 601 TACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTC 660
| |
| − |
| |
| − |
| |
| − | Query 807 CTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG 863
| |
| − | Sbjct 661 CTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG 717
| |
| − |
| |
| − | The Part YFP part which was designed carried the N-terminal fusion tag.
| |
| | | | |
| | ===Source=== | | ===Source=== |
