Difference between revisions of "Part:BBa K5175009:Design"

 
 
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===Design Notes===
 
===Design Notes===
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TphB is a dehydrogenase that oxidizes the diol moiety (two hydroxyl groups) of DCD to a keto group, resulting in the production of PCA.<br>
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The tph gene cluster (tphA2-tphA3-tphB-tphA1, tpaK) was introduced for the complete uptake and utilization of terephthalic acid (TPA).<br>
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===Source===
 
===Source===
  
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Comamonas sp.E6
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<i>Comamonas sp.</i> E6
  
 
===References===
 
===References===
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[1] SASOH M, MASAI E, ISHIBASHI S, et al. Characterization of the Terephthalate Degradation Genes of <i>Comamonas sp.</i> Strain E6 [J]. Applied and Environmental Microbiology, 2006, 72(3): 1825-32.<br>
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[2] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.<br>

Latest revision as of 07:03, 2 October 2024


tphB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TphB is a dehydrogenase that oxidizes the diol moiety (two hydroxyl groups) of DCD to a keto group, resulting in the production of PCA.
The tph gene cluster (tphA2-tphA3-tphB-tphA1, tpaK) was introduced for the complete uptake and utilization of terephthalic acid (TPA).

Source

Comamonas sp. E6

References

[1] SASOH M, MASAI E, ISHIBASHI S, et al. Characterization of the Terephthalate Degradation Genes of Comamonas sp. Strain E6 [J]. Applied and Environmental Microbiology, 2006, 72(3): 1825-32.
[2] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.