Difference between revisions of "Part:BBa K5291049:Design"
 
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===Design Notes===
 
===Design Notes===
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In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB<sub>2</sub>-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB<sub>2</sub>-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AdhA is a significant component in degradation module.
+
In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AdhA is a significant component in degradation module.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Chen, S., et al. (2024). "An NAD(+)-dependent group Ⅲ alcohol dehydrogenase involved in long-chain alkane degradation in Acinetobacter venetianus RAG-1." Enzyme Microb Technol 172: 110343.

Latest revision as of 07:00, 2 October 2024


AdhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AdhA is a significant component in degradation module.

Source

Acinetobacter venetianus RAG-1

References

Chen, S., et al. (2024). "An NAD(+)-dependent group Ⅲ alcohol dehydrogenase involved in long-chain alkane degradation in Acinetobacter venetianus RAG-1." Enzyme Microb Technol 172: 110343.