Difference between revisions of "Part:BBa K257024:Design"

 
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<partinfo>BBa_K257024 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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We designed oligo to mutate Jun so it could no more form homodimere
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We designed primers to introduce a mutation in Jun so it could no more form homodimeres.
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This part needs to have silver standard fusion 23 at both N term and Cterm. Indeed, we use it as a passenger in an autotransporter that allows us to present jun at the outer side of the outer membrane of the cell. We designed a protein fusion with : signal peptide Sec-depedent to export jun from cytoplasm to periplasm + the passenger protein (jun)+ the linker and translocator domain of AIDA which will be anchored in the outer membrane.
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===Source===
 
===Source===
  
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DNA
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DNA was synthetized in vitro.
  
 
===References===
 
===References===

Latest revision as of 08:37, 30 October 2009

Jun : first part of the dimere Jun/Fos


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers to introduce a mutation in Jun so it could no more form homodimeres. This part needs to have silver standard fusion 23 at both N term and Cterm. Indeed, we use it as a passenger in an autotransporter that allows us to present jun at the outer side of the outer membrane of the cell. We designed a protein fusion with : signal peptide Sec-depedent to export jun from cytoplasm to periplasm + the passenger protein (jun)+ the linker and translocator domain of AIDA which will be anchored in the outer membrane.

Source

DNA was synthetized in vitro.

References