Difference between revisions of "Part:BBa K5115004"

Latest revision as of 06:53, 2 October 2024

hoxY

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115013(ribozyme+RBS+hoxY+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 57
Illegal XhoI site found at 264
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 357
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 __TOC__
 ===Introduction===
-The hoxU is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
+The hoxY is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
 ===Usage and Biology===
-It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was construct into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115013]
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115013 BBa_K5115013(ribozyme+RBS+hoxY+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
+===Sequence and Features===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>