Difference between revisions of "Part:BBa K257021"
 
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<partinfo>BBa_K257021 short</partinfo>
 
<partinfo>BBa_K257021 short</partinfo>
  
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It is the second part of our delay system test. The first part is PBad+RBS-LacI-LVA + RBS + YFP + Ter.
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This part can be used to introduce a delay between induction and expression of a desired gene.
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It is another possibility for our delay system test. The first part of this delay test is PBad+RBS-LacI-LVA + RBS + YFP + Ter.
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K257021 parameters</partinfo>
 
<partinfo>BBa_K257021 parameters</partinfo>
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====Measurement of BBa_K257021 delay====
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BBa_K257021 was grown overnight in LB medium with:<br>
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- Glucose 1 % <br>
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- nothing <br>
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- IPTG (0.2mg/ml) <br>
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Each of the 3 overnight cultures was then diluted to the 1/100 in each of the 3 conditions. Fluorescence and OD were assessed in a Tecan plate reader.
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[[Image:PTPR-Fluo OD.JPG|450px]][[Image:PTPR-Normalized Fluo OD.JPG|450px]]
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In the second graph, fluorescence was normalized by the mean Fluo/OD of all the conditions where mRFP1 is continuously expressed.
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We observe a delay of ~10000sec between the beginning of the experiment and the changes in RFP expression.
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Note that the delay is shorter for the LB + glucose -> LB + IPTG shift than for the LB + glucose -> LB shift.

Latest revision as of 08:21, 30 October 2009

PLacI + RBS-TetR + Ter + Ptet + RFP

This part can be used to introduce a delay between induction and expression of a desired gene.

It is another possibility for our delay system test. The first part of this delay test is PBad+RBS-LacI-LVA + RBS + YFP + Ter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1495
    Illegal AgeI site found at 1607
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aPLacI + RBS-TetR + Ter + Ptet + RFP

Measurement of BBa_K257021 delay

BBa_K257021 was grown overnight in LB medium with:
- Glucose 1 %
- nothing
- IPTG (0.2mg/ml)

Each of the 3 overnight cultures was then diluted to the 1/100 in each of the 3 conditions. Fluorescence and OD were assessed in a Tecan plate reader. PTPR-Fluo OD.JPGPTPR-Normalized Fluo OD.JPG

In the second graph, fluorescence was normalized by the mean Fluo/OD of all the conditions where mRFP1 is continuously expressed.

We observe a delay of ~10000sec between the beginning of the experiment and the changes in RFP expression.

Note that the delay is shorter for the LB + glucose -> LB + IPTG shift than for the LB + glucose -> LB shift.