Difference between revisions of "Part:BBa K5115001"
 
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<partinfo>BBa_K5115001 short</partinfo>
 
<partinfo>BBa_K5115001 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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The Ni-Fe hydrogenase we use is an enzyme that can catalyze not only the reduction of hydrogen ions to hydrogen under anaerobic conditions but also the reversed reaction under aerobic conditions. Among this enzyme, hoxF is a hydrogenase subunit responsible for electron transport, which, together with the hoxU subunit, forms the module of NADH dehydrogenase.
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The hoxF is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
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===Usage and Biology===
 
===Usage and Biology===
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HoxF subunit is very important in Ni-Fe hydrogenase  
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It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115011 BBa_K5115011(ribozyme+RBS+hoxF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
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The most important group in hoxF is FMN-b, which has the ability of switching electron. Under anaerobic conditions, NADH is oxidized to NAD<sup>+</sup> on the surface of hoxF subunit. Under aerobic conditions, NAD<sup>+</sup> is reduced to NADH on the surface of the hoxF subunit. Under anaerobic conditions, the electrons generated in this reaction travel through a series of processes to the hoxH, completing the final reduction of the hydrogen ion.
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===Characterization===
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 06:19, 2 October 2024


hoxF, without stop codon

contributed by Fudan iGEM 2024

Introduction

The hoxF is a subunit of the hox and hyp operon BBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115011(ribozyme+RBS+hoxF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 577
    Illegal BglII site found at 1395
    Illegal BglII site found at 1688
    Illegal XhoI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 994
    Illegal NgoMIV site found at 1204
    Illegal NgoMIV site found at 1516
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 625
    Illegal BsaI.rc site found at 1129


References