Difference between revisions of "Part:BBa K5499013"
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We constructed the IDS coding gene downstream of the strong CMV promoter, with the aim that, after transfecting HEK293T cells with the lentiviral expression vector containing this composite part, the cells would stably and abundantly express IDS. | We constructed the IDS coding gene downstream of the strong CMV promoter, with the aim that, after transfecting HEK293T cells with the lentiviral expression vector containing this composite part, the cells would stably and abundantly express IDS. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
| − | === | + | ===Characterization=== |
| + | We constructed this composite part into a lentiviral expression vector and then transfected it into HEK293T cells. Subsequently, we extracted total protein and exosomes from the cells and performed Western Blotting analysis. | ||
| + | As shown in Figure 1, the experimental group exhibited high levels of IDS expression in the cells compared to the blank control group (left panel). However, there was no significant difference in the expression levels of IDS in the exosomes between the experimental group and the blank control group (right panel). | ||
| + | <html> | ||
| + | <figure style="text-align:center;"> | ||
| + | <img style="max-width:700px;" src="https://static.igem.wiki/teams/5499/registry-wang/wb-for-1st-genetation.png" alt="control"> | ||
| + | <figcaption><b>（Figure1:</b> Left Panel) Western Blotting Detection of Protein Content in cell Right Panel) Western Blotting Detection of Protein Content in Exosomes</figcaption> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 04:05, 2 October 2024
Engineered exosome 1
We constructed the IDS coding gene downstream of the strong CMV promoter, with the aim that, after transfecting HEK293T cells with the lentiviral expression vector containing this composite part, the cells would stably and abundantly express IDS.
Characterization
We constructed this composite part into a lentiviral expression vector and then transfected it into HEK293T cells. Subsequently, we extracted total protein and exosomes from the cells and performed Western Blotting analysis.
As shown in Figure 1, the experimental group exhibited high levels of IDS expression in the cells compared to the blank control group (left panel). However, there was no significant difference in the expression levels of IDS in the exosomes between the experimental group and the blank control group (right panel).
