Difference between revisions of "Part:BBa K243034"
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[[Image:Freiburg09 pJS418.JPG|550x450px]] | [[Image:Freiburg09 pJS418.JPG|550x450px]] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. | + | The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance of the start codon to the included ribosome binding site, some restriction sites of the assembly standard 25 have been avoided. |
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Latest revision as of 19:41, 23 October 2009
pJS418
Expression vector with a strong Shine-Dalgarno sequence designed for E. coli expression strains.
Usage and Biology
The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance of the start codon to the included ribosome binding site, some restriction sites of the assembly standard 25 have been avoided.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 2267
Illegal XbaI site found at 1383 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2268
Illegal PstI site found at 2282
Illegal NotI site found at 2275 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1495
Illegal BamHI site found at 2366 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2268
Illegal XbaI site found at 1383 - 25INCOMPATIBLE WITH RFC[25]Illegal suffix found in sequence at 2258
Illegal XbaI site found at 1383
Illegal NgoMIV site found at 1391
Illegal NgoMIV site found at 2499
Illegal AgeI site found at 3833 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2115