Difference between revisions of "Part:BBa K243034"
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<partinfo>BBa_K243034 short</partinfo>
 
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Expression vector with a strong Shine-Dalgarno sequence designed for E. coli expression strains. <br>
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===Usage and Biology===
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[[Image:Freiburg09 pJS418.JPG|550x450px]]
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===Usage and Biology===
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The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance of the start codon to the included ribosome binding site, some restriction sites of the assembly standard 25 have been avoided.
 
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243034 SequenceAndFeatures</partinfo>

Latest revision as of 19:41, 23 October 2009

pJS418


Expression vector with a strong Shine-Dalgarno sequence designed for E. coli expression strains.


Freiburg09 pJS418.JPG

Usage and Biology

The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance of the start codon to the included ribosome binding site, some restriction sites of the assembly standard 25 have been avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2267
    Illegal XbaI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2268
    Illegal PstI site found at 2282
    Illegal NotI site found at 2275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1495
    Illegal BamHI site found at 2366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2268
    Illegal XbaI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2258
    Illegal XbaI site found at 1383
    Illegal NgoMIV site found at 1391
    Illegal NgoMIV site found at 2499
    Illegal AgeI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2115