Difference between revisions of "Part:BBa K243034"

Revision as of 19:40, 23 October 2009

pJS418

Expression vector with a strong Shine-Dalgarno sequence designed for E. coli expression strains.

Usage and Biology

The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance to the included ribosome binding site, not all restriction sites of the assembly standard 25 are included.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 2267
Illegal XbaI site found at 1383
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 2268
Illegal PstI site found at 2282
Illegal NotI site found at 2275
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1495
Illegal BamHI site found at 2366
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 2268
Illegal XbaI site found at 1383
25
INCOMPATIBLE WITH RFC[25]
Illegal suffix found in sequence at 2258
Illegal XbaI site found at 1383
Illegal NgoMIV site found at 1391
Illegal NgoMIV site found at 2499
Illegal AgeI site found at 3833
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2115

@@ Line 8: / Line 8: @@
 [[Image:Freiburg09 pJS418.JPG|550x450px]]
 ===Usage and Biology===
-The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection.
+The pJS419 vector is an expression vector with a strong RBS, it is useful for the expression of non-toxic genes and products which do not easily aggregate at a high expression level. The plasmid confers chloramphenicol resistance to the host cell allowing a positive selection. The picture shows an inserted ampicillin resistance gene and the flanking restriction sites. Due to the limited distance to the included ribosome binding site, not all restriction sites of the assembly standard 25 are included.
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