Difference between revisions of "Part:BBa K5233013:Design"

 
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===Design Notes===
 
===Design Notes===
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MHET is extracellular, and we need to use Amcut to secrete MHETase out of the cell to degrade MHET.
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The MHETase is used for degrading MHET to TPA and promoting the whole process of degradation of PET. Since it is also connected with pelB siganl peptide, it can be secreted outside the bacteria to function.
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===Source===
 
===Source===
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===References===
 
===References===
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Janatunaim RZ, Fibriani A. Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase). Recent Pat Biotechnol. 2020;14(3):229-234. doi: 10.2174/1872208314666200311104541. PMID: 32160855.

Latest revision as of 03:18, 2 October 2024


MHETase(PelB), a kind of tannase family protein that can degrade MHET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1478
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1478
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1478
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 451
    Illegal AgeI site found at 1101
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MHETase is used for degrading MHET to TPA and promoting the whole process of degradation of PET. Since it is also connected with pelB siganl peptide, it can be secreted outside the bacteria to function.

Source

It comes from A. mediterranei

References

Janatunaim RZ, Fibriani A. Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase). Recent Pat Biotechnol. 2020;14(3):229-234. doi: 10.2174/1872208314666200311104541. PMID: 32160855.