Difference between revisions of "Part:BBa K5079030:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part's insertion site has a free energy of 1.1. The insertion's residue has a SASA of 57%. The residue is conserved. This protein switch has not gone through circular permutation. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 16 and the loop distance is 20.6. The linker itself is 3 residues long, composed of "GPG".
  
  
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===Source===
 
===Source===
  
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===References===
 
===References===

Latest revision as of 02:52, 2 October 2024


Protein Switch 5 (K_45_WT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part's insertion site has a free energy of 1.1. The insertion's residue has a SASA of 57%. The residue is conserved. This protein switch has not gone through circular permutation. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 16 and the loop distance is 20.6. The linker itself is 3 residues long, composed of "GPG".


Source

References