Difference between revisions of "Part:BBa K243031"
| Line 6: | Line 6: | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
This plasmid has been used to assemble and clone a variety of nucleotide sequences. As the assembly standard allows it, the creation of fusion proteins is easily achieved. The vector confers resistance towards ampicillin to the E. coli host cell. Since the inserted part is not expressed, toxic proteins can be cloned and combined. | This plasmid has been used to assemble and clone a variety of nucleotide sequences. As the assembly standard allows it, the creation of fusion proteins is easily achieved. The vector confers resistance towards ampicillin to the E. coli host cell. Since the inserted part is not expressed, toxic proteins can be cloned and combined. | ||
Latest revision as of 18:19, 22 October 2009
pMA
This plasmid was used by the Freiburg team for construction and cloning of parts according to the BioBrick Assembly standard 25.
Usage and Biology
This plasmid has been used to assemble and clone a variety of nucleotide sequences. As the assembly standard allows it, the creation of fusion proteins is easily achieved. The vector confers resistance towards ampicillin to the E. coli host cell. Since the inserted part is not expressed, toxic proteins can be cloned and combined.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1988
Illegal suffix found in sequence at 2038 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1988
Illegal SpeI site found at 2039
Illegal PstI site found at 2053
Illegal NotI site found at 1994
Illegal NotI site found at 2046 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1988
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1988
Illegal suffix found in sequence at 2039 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1988
Illegal suffix found in sequence at 2029 - 1000COMPATIBLE WITH RFC[1000]