Difference between revisions of "Part:BBa K243031"
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This is a plasmid that we use for consruction and further cloning steps.
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This plasmid was used by the Freiburg team for construction and cloning of parts according to the BioBrick Assembly standard 25.
  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This plasmid has been used to assemble and clone a variety of nucleotide sequences. As the assembly standard allows it, the creation of fusion proteins is easily achieved. The vector confers resistance towards ampicillin to the E. coli host cell. Since the inserted part is not expressed, toxic proteins can be cloned and combined.
  
 
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Revision as of 18:19, 22 October 2009

pMA


This plasmid was used by the Freiburg team for construction and cloning of parts according to the BioBrick Assembly standard 25.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1988
    Illegal SpeI site found at 2039
    Illegal PstI site found at 2053
    Illegal NotI site found at 1994
    Illegal NotI site found at 2046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1988
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2029
  • 1000
    COMPATIBLE WITH RFC[1000]