Difference between revisions of "Part:BBa K243030"
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<partinfo>BBa_K243030 short</partinfo> | <partinfo>BBa_K243030 short</partinfo> | ||
| − | This part is a linker, it can be used to connect two parts and add additional space between | + | This part is a peptide linker, it can be used to connect two protein parts / domains and add additional space between them. This can be necessary to avoid interactions between the different parts when fusion proteins are created. The SEG linker was created to combine our protein domains [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243000 Fok_a] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243001 Fok_i] to a fusion construct. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Peptide linkers are used to connect two peptides. In nature, many of them connect individual domains of a protein. They are often flexible and vary in length. Especially for the creation of artificial fusion proteins, it is important for this connection that the linker itself has no influence on the connected peptides. Therefore the sequence of the linker is designed with amino acids which do not interact with amino acids in their environment. The amino acids glycine and serine are zwitterionic and hydrophile - these properties make them a good choice for the repetitive sequence of the linker.<br> | |
| − | The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short there might be a sterical interference between the parts and if it is too long, it can cause | + | The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short, there might be a sterical interference between the parts and if it is too long, it can cause instability of the construct. It is also important that the linker has a certain flexibility although too much flexibility may interfere with the function of the linked peptides. <br> <br> |
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Latest revision as of 18:04, 22 October 2009
SEG
This part is a peptide linker, it can be used to connect two protein parts / domains and add additional space between them. This can be necessary to avoid interactions between the different parts when fusion proteins are created. The SEG linker was created to combine our protein domains Fok_a and Fok_i to a fusion construct.
Usage and Biology
Peptide linkers are used to connect two peptides. In nature, many of them connect individual domains of a protein. They are often flexible and vary in length. Especially for the creation of artificial fusion proteins, it is important for this connection that the linker itself has no influence on the connected peptides. Therefore the sequence of the linker is designed with amino acids which do not interact with amino acids in their environment. The amino acids glycine and serine are zwitterionic and hydrophile - these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short, there might be a sterical interference between the parts and if it is too long, it can cause instability of the construct. It is also important that the linker has a certain flexibility although too much flexibility may interfere with the function of the linked peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]