Difference between revisions of "Part:BBa K243027"
 
(16 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K243027 short</partinfo>[[Image:Image:1fos bio r 250.jpg]]
+
<partinfo>BBa_K243027 short</partinfo><br>
 +
 
 +
This part is composed of a polyhistidine-tagged leucine zipper domain derived from the mammalian c-Fos protein. It can form DNA-binding homo- and heterodimers and can be used to target fused protein parts to specific DNA regions.
 +
<br>
 +
[[Image:1fos bio r 250.jpg]] <br>
 +
[http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS Heterodimeric Fos complex]
  
−
The heterodimeric Fos complex is a transcriptional activator involved in the signal transduction pathway. Via leucin zipper they interact among each other and with their bipartite domain, rich of basic amino acids, they bind DNA (Abate et al., Mol Cell Biol. 1991 July).
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers and binds DNA as a dimer. Two classes of core DNA sequences, the sequences TRE (TGACTCA) and CRE (TGACGTCA), can be recognized by AP-1. Nine homologues of the AP-1 leucine zipper region have been characterized. All of them are able to form heterodimers, some also form homodimers. One of them is the so called c-Fos. Via their leucin zipper domains they interact with each other and bind DNA with their bipartite domain (Abate et al., Mol Cell Biol., 1991). The sequence for the bZip domains of c-Fos is linked to a His tag which allows protein purification using a Ni-NTA column. Soluble expression in ''Escherichia coli'' is possible.<br>
 +
In the research dimerized c-fos is normally used as a DNA binding molecule.<br>
 +
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243027 SequenceAndFeatures</partinfo>
  

Latest revision as of 17:30, 22 October 2009

His-FOS

This part is composed of a polyhistidine-tagged leucine zipper domain derived from the mammalian c-Fos protein. It can form DNA-binding homo- and heterodimers and can be used to target fused protein parts to specific DNA regions.
1fos bio r 250.jpg
[http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS Heterodimeric Fos complex]


Usage and Biology

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers and binds DNA as a dimer. Two classes of core DNA sequences, the sequences TRE (TGACTCA) and CRE (TGACGTCA), can be recognized by AP-1. Nine homologues of the AP-1 leucine zipper region have been characterized. All of them are able to form heterodimers, some also form homodimers. One of them is the so called c-Fos. Via their leucin zipper domains they interact with each other and bind DNA with their bipartite domain (Abate et al., Mol Cell Biol., 1991). The sequence for the bZip domains of c-Fos is linked to a His tag which allows protein purification using a Ni-NTA column. Soluble expression in Escherichia coli is possible.
In the research dimerized c-fos is normally used as a DNA binding molecule.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]