Difference between revisions of "Part:BBa K208005"

Latest revision as of 16:45, 22 October 2009

TorA Signal Peptide - Silver Fusion Compatible

This is a Silver-fusion compatible BioBrick part that can be attached to other proteins to target those proteins for export out of the cytoplasm. The TorA sequence targets folded proteins to the Tat-dependent pathway of Type II secretion.

Usage and Biology

The TorA signal peptide has been used by multiple researchers to export folded GFP into the periplasm of cells (Barrett, 2003; Santini, 2001; Thomas, 2001). It can be fused to non-exportable proteins as a tag for translocation out of the periplasm by the Tat-dependent system.

The Twin-arginine-translcocation system is used to export folded proteins into the periplasmic space (Choi, 2004). Like the Sec-dependent pathways, specific N-terminal signal peptide sequences target a protein for export by the TAT machinery. Although similar, TAT signal peptides differ from those that target proteins to the Sec machinery. TAT signal peptides contain a conserved sequence of seven amino acids, (S/T)-R-R-x-F-L-K, at the interface between the N- and H-regions, where x represents a polar amino acid (Berks, 2000; Palmer, 2004). The twin-arginine residues are consistently present in TAT signal peptides, and the occurrence of the other amino acids is greater than 50% (Berks 1996, Berks 2000, Palmer, 2004). The qualities, such as hydrophobicity, affect the secretion mechanism for which it targets. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K208005 short</partinfo>
-torA
+This is a Silver-fusion compatible BioBrick part that can be attached to other proteins to target those proteins for export out of the cytoplasm. The TorA sequence targets folded proteins to the Tat-dependent pathway of Type II secretion.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The TorA signal peptide has been used by multiple researchers to export folded GFP into the periplasm of cells (Barrett, 2003; Santini, 2001; Thomas, 2001). It can be fused to non-exportable proteins as a tag for translocation out of the periplasm by the Tat-dependent system.
+The Twin-arginine-translcocation system is used to export folded proteins into the periplasmic space (Choi, 2004). Like the Sec-dependent pathways, specific N-terminal signal peptide sequences target a protein for export by the TAT machinery. Although similar, TAT signal peptides differ from those that target proteins to the Sec machinery. TAT signal peptides contain a conserved sequence of seven amino acids, (S/T)-R-R-x-F-L-K, at the interface between the N- and H-regions, where x represents a polar amino acid (Berks, 2000; Palmer, 2004). The twin-arginine residues are consistently present in TAT signal peptides, and the occurrence of the other amino acids is greater than 50% (Berks 1996, Berks 2000, Palmer, 2004). The qualities, such as hydrophobicity, affect the secretion mechanism for which it targets.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>