Difference between revisions of "Part:BBa K208003:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
OmpA secretion tag.  Silver fusion compatible.  In pSB1A2.
+
Silver-fusion compatible OmpA secretion tag in pSB1A2
  
 
===Source===
 
===Source===
  
−
Anneal
+
To construct the OmpA sequence, complimentary forward and reverse oligonucleotides were synthesized. These strands were then annealed together. The oligonucleotides were designed so that the Silver fusion prefix and suffix sequences were appended onto the end of the sequence. This parts was then cut with EcoRI and SpeI and ligated into a BioBrick vector.
  
 
===References===
 
===References===
 +
1. Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli Appl Microbiol Biotechnol 64:625-635<br>
 +
2. Luirink J, Oudega B (2004) Protein targeting to the inner membrane. In: Oudega B, editor. Kluwer Academic Publishers, pp 1-21<br>
 +
3. Molhoj M (2004) Leader sequences are not signal peptides. Nat Biotech 22:1502<br>
 +
4. Pugsley AP (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57:50-108 <br>
 +
5. van der Does C, Noewen N, Driessen AJM (2004) The Sec translocase. In: Oudega B, editor. Kluwer Academic Publishers, pp:23-49<br>
 +
6. Veenendaal AKL, van der Does C, Driessen AJM (2004) The protein-conducting channel SecYEG. Biochimica et Biophysica Act 1694:81-95<br>
 +
7. von Heijne G (1986) Net N-C charge imbalance may be important for signal sequence function in bacteria. J Mol Biol 192:287-290

Latest revision as of 16:24, 22 October 2009

OmpA Signal Peptide - Silver Fusion Compatible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silver-fusion compatible OmpA secretion tag in pSB1A2

Source

To construct the OmpA sequence, complimentary forward and reverse oligonucleotides were synthesized. These strands were then annealed together. The oligonucleotides were designed so that the Silver fusion prefix and suffix sequences were appended onto the end of the sequence. This parts was then cut with EcoRI and SpeI and ligated into a BioBrick vector.

References

1. Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli Appl Microbiol Biotechnol 64:625-635
2. Luirink J, Oudega B (2004) Protein targeting to the inner membrane. In: Oudega B, editor. Kluwer Academic Publishers, pp 1-21
3. Molhoj M (2004) Leader sequences are not signal peptides. Nat Biotech 22:1502
4. Pugsley AP (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57:50-108
5. van der Does C, Noewen N, Driessen AJM (2004) The Sec translocase. In: Oudega B, editor. Kluwer Academic Publishers, pp:23-49
6. Veenendaal AKL, van der Does C, Driessen AJM (2004) The protein-conducting channel SecYEG. Biochimica et Biophysica Act 1694:81-95
7. von Heijne G (1986) Net N-C charge imbalance may be important for signal sequence function in bacteria. J Mol Biol 192:287-290