Difference between revisions of "Part:BBa K208010:Design"

(→‎Design Notes)
(→‎Source)
 
(2 intermediate revisions by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Lac promoter with a RBS.  In pSB1AK3.
+
This Lac promoter with an attached RBS was constructed in pSB1AK3. There are no additional design parameters that need to be considered.
  
 
===Source===
 
===Source===
  
−
synthetic
+
This part was constructed using existing parts in the BioBrick registry. For simplicity, the USU iGEM team designed primers to isolate this entire promoter/rbs sequence out of an existing composite part, BBa_K084007. The primers were designed so that the biobrick prefix and suffix were overhanging. The resulting PCR product was then cut and ligated into a BioBrick compatible vector, pSB1AK3. This part has been sequenced and is correct.
  
 
===References===
 
===References===

Latest revision as of 15:36, 22 October 2009

Lac Promoter (BBa_R0010) and RBS (B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Lac promoter with an attached RBS was constructed in pSB1AK3. There are no additional design parameters that need to be considered.

Source

This part was constructed using existing parts in the BioBrick registry. For simplicity, the USU iGEM team designed primers to isolate this entire promoter/rbs sequence out of an existing composite part, BBa_K084007. The primers were designed so that the biobrick prefix and suffix were overhanging. The resulting PCR product was then cut and ligated into a BioBrick compatible vector, pSB1AK3. This part has been sequenced and is correct.

References