Difference between revisions of "Part:BBa K243037"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K243037 short</partinfo><br><br>
 
<partinfo>BBa_K243037 short</partinfo><br><br>
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The cloning vector pBAD has the RFC25 restriction sites and an ampicilin resistance. It is inducible by arabinose and when the inserted part has an RBS, it can be used as an expression vector. <br><br>
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The cloning vector pBAD carries the RFC25 restriction sites and an ampicilin resistance. It can be used as an expression vector if the inserted BioBrick contains a ribosome binding site. In this case, expression of the protein of interest can be induced by addition of arabinose. <br><br>
 
[[Image:PBADvetocorplasmapper.png|550x550px]]<br>
 
[[Image:PBADvetocorplasmapper.png|550x550px]]<br>
 
plasMap
 
plasMap
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===Usage and Biology===
 
===Usage and Biology===
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The protein expression starts only when induced by arabinose, so this vector is useful for the expression of toxic products.<br>
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Protein expression is only enabled when the inserted coding sequence possesses a ribosome binding site and arabinose is added to the cells, rendering this vector useful for cloning and expression of genes toxic for E. coli.<br>
  
  

Latest revision as of 14:34, 22 October 2009

pBAD

The cloning vector pBAD carries the RFC25 restriction sites and an ampicilin resistance. It can be used as an expression vector if the inserted BioBrick contains a ribosome binding site. In this case, expression of the protein of interest can be induced by addition of arabinose.

PBADvetocorplasmapper.png
plasMap


Usage and Biology

Protein expression is only enabled when the inserted coding sequence possesses a ribosome binding site and arabinose is added to the cells, rendering this vector useful for cloning and expression of genes toxic for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2434
    Illegal SpeI site found at 3052
    Illegal PstI site found at 3066
    Illegal NotI site found at 2440
    Illegal NotI site found at 3059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2434
    Illegal BamHI site found at 2338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3052
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3106
    Illegal BsaI site found at 3284
    Illegal BsaI site found at 4352
    Illegal SapI site found at 2155
    Illegal SapI.rc site found at 783
    Illegal SapI.rc site found at 2949