Difference between revisions of "Part:BBa K5044311"

 
 
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<partinfo>BBa_K5044311 short</partinfo>
 
<partinfo>BBa_K5044311 short</partinfo>
  
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Description:
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rrnB terminator from Escherichia coli
  
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pYY11 is an intermediate plasmid that served as a crucial precursor in the development of the pQQC7 plastid transformation vector. This plasmid was designed and constructed to incorporate essential components and regulatory elements that were later refined and integrated into the final pQQC7 construct.
+
<!-- Add more about the biology of this part here
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===Usage and Biology===
  
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Key Features and Role:
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<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5044311 SequenceAndFeatures</partinfo>
  
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Foundation for pQQC7:
 
−
pYY11 provided the initial backbone and key elements that were further optimized and modified to create pQQC7. It served as a testing ground for various components, ensuring their functionality and compatibility before final integration.
 
−
Cloning Flexibility:
 
−
pYY11 includes multiple restriction enzyme sites, allowing for easy insertion and manipulation of genes and regulatory elements. This flexibility was crucial for the iterative design and testing phases of pQQC7.
 
−
Regulatory Elements:
 
−
The plasmid contains preliminary versions of the promoters, 5' UTRs, and other regulatory sequences that were later fine-tuned in pQQC7. These elements were tested for their expression levels and stability in the chloroplast environment.
 
−
Selectable Marker and Reporter Gene:
 
−
pYY11 includes the aadA selectable marker gene and the GFP reporter gene, which were used to test and validate the selection and visualization processes. These genes were driven by early versions of the psbA and Prrn promoters, respectively.
 
−
Homologous Recombination Sequences:
 
−
Initial homologous recombination regions (LHRR and RHRR) were included in pYY11 to ensure proper integration into the kiwifruit chloroplast genome. These sequences were later refined and optimized in pQQC7.
 
−
Stability and Compatibility:
 
−
pYY11 was designed to be stable and compatible with standard molecular biology techniques, ensuring that it could be efficiently propagated and maintained in bacterial hosts.
 
−
Summary:
 
  
−
pYY11 is an intermediate plasmid that played a vital role in the development of the pQQC7 plastid transformation vector. It provided the foundational structure and key components that were further refined and optimized to create the final, highly functional pQQC7 plasmid. This intermediate step was essential for ensuring the robustness and reliability of the final construct, making pYY11 a critical part of our project's success.
+
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K5044311 parameters</partinfo>
 +
<!-- -->
  
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Usage:
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<html lang="en">
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<head>
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    <meta charset="UTF-8">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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    <title>pYY11 - Intermediate Plasmid for pQQC7 Development</title>
  
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Development and Testing: pYY11 can be used as a reference or starting point for researchers who are interested in the development and optimization of plastid transformation vectors.
+
 
−
Educational Tool: It serves as an educational tool to demonstrate the iterative process of plasmid design and the importance of intermediate steps in the development of advanced genetic constructs.
+
__NOTOC__
 +
<partinfo>BBa_K5044302 short</partinfo>
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 +
rrnB terminator from Escherichia coli
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5044311 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K5044302 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K5044311 parameters</partinfo>
+
<partinfo>BBa_K5044302 parameters</partinfo>
 
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    <style>
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        body {
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            font-family: Arial, sans-serif;
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            margin: 20px;
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            line-height: 1.6;
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            text-align: center;
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            margin: 20px 0;
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        }
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        .image-container img {
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            max-width: 100%;
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            height: auto;
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            border: 1px solid #ddd;
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            border-radius: 4px;
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            padding: 5px;
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            box-shadow: 0 2px 4px rgba(0, 0, 0, 0.1);
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        }
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    </style>
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</head>
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<body>
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<h1>pYY11 - Intermediate Plasmid for pQQC7 Development</h1>
 +
 +
<partinfo>BBa_K5044311 short</partinfo>
 +
 +
<div class="section">
 +
    <h2>Description:</h2>
 +
 +
    <p>pYY11 is an intermediate plasmid that served as a crucial precursor in the development of the pQQC7 plastid transformation vector. This plasmid was designed and constructed to incorporate essential components and regulatory elements that were later refined and integrated into the final pQQC7 construct.</p>
 +
</div>
 +
 +
 +
<!-- Image Section -->
 +
<div class="section image-container">
 +
    <h2>Plasmid Map of pYY11</h2>
 +
    <img src="https://static.igem.wiki/teams/5044/pyy11.jpg" alt="Plasmid Map of pYY11">
 +
</div>
 +
 +
 +
<div class="section">
 +
    <h2>Key Features and Role:</h2>
 +
    <ul>
 +
        <li><strong>Foundation for pQQC7:</strong> pYY11 provided the initial backbone and key elements that were further optimized and modified to create pQQC7. It served as a testing ground for various components, ensuring their functionality and compatibility before final integration.</li>
 +
        <li><strong>Cloning Flexibility:</strong> pYY11 includes multiple restriction enzyme sites, allowing for easy insertion and manipulation of genes and regulatory elements. This flexibility was crucial for the iterative design and testing phases of pQQC7.</li>
 +
        <li><strong>Regulatory Elements:</strong> The plasmid contains preliminary versions of the promoters, 5' UTRs, and other regulatory sequences that were later fine-tuned in pQQC7. These elements were tested for their expression levels and stability in the chloroplast environment.</li>
 +
        <li><strong>Selectable Marker and Reporter Gene:</strong> pYY11 includes the aadA selectable marker gene and the GFP reporter gene, which were used to test and validate the selection and visualization processes. These genes were driven by early versions of the psbA and Prrn promoters, respectively.</li>
 +
        <li><strong>Homologous Recombination Sequences:</strong> Initial homologous recombination regions (LHRR and RHRR) were included in pYY11 to ensure proper integration into the kiwifruit chloroplast genome. These sequences were later refined and optimized in pQQC7.</li>
 +
        <li><strong>Stability and Compatibility:</strong> pYY11 was designed to be stable and compatible with standard molecular biology techniques, ensuring that it could be efficiently propagated and maintained in bacterial hosts.</li>
 +
    </ul>
 +
</div>
 +
 +
<div class="section">
 +
    <h2>Summary:</h2>
 +
    <p>pYY11 is an intermediate plasmid that played a vital role in the development of the pQQC7 plastid transformation vector. It provided the foundational structure and key components that were further refined and optimized to create the final, highly functional pQQC7 plasmid. This intermediate step was essential for ensuring the robustness and reliability of the final construct, making pYY11 a critical part of our project's success.</p>
 +
</div>
 +
 +
<div class="section">
 +
    <h2>Usage:</h2>
 +
    <ul>
 +
        <li><strong>Development and Testing:</strong> pYY11 can be used as a reference or starting point for researchers who are interested in the development and optimization of plastid transformation vectors.</li>
 +
        <li><strong>Educational Tool:</strong> It serves as an educational tool to demonstrate the iterative process of plasmid design and the importance of intermediate steps in the development of advanced genetic constructs.</li>
 +
    </ul>
 +
</div>
 +
 +
<!-- Add more about the biology of this part here -->
 +
<div class="section">
 +
    <h2>Sequence and Features</h2>
 +
    <partinfo>BBa_K5044311 SequenceAndFeatures</partinfo>
 +
</div>
 +
 +
<!-- Uncomment this to enable Functional Parameter display -->
 +
<!--
 +
<div class="section">
 +
    <h2>Functional Parameters</h2>
 +
    <partinfo>BBa_K5044311 parameters</partinfo>
 +
</div>
 +
-->
 +
 +
</body>
 +
</html>

Latest revision as of 01:28, 2 October 2024


pYY11: Predecessor Plasmid for pQQC7

rrnB terminator from Escherichia coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 895
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2056
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2301
    Illegal BsaI.rc site found at 813


pYY11 - Intermediate Plasmid for pQQC7 Development __NOTOC__ BBa_K5044302 short rrnB terminator from Escherichia coli Sequence and Features BBa_K5044302 SequenceAndFeatures

pYY11 - Intermediate Plasmid for pQQC7 Development

BBa_K5044311 short

Description:

pYY11 is an intermediate plasmid that served as a crucial precursor in the development of the pQQC7 plastid transformation vector. This plasmid was designed and constructed to incorporate essential components and regulatory elements that were later refined and integrated into the final pQQC7 construct.

Plasmid Map of pYY11

Plasmid Map of pYY11

Key Features and Role:

  • Foundation for pQQC7: pYY11 provided the initial backbone and key elements that were further optimized and modified to create pQQC7. It served as a testing ground for various components, ensuring their functionality and compatibility before final integration.
  • Cloning Flexibility: pYY11 includes multiple restriction enzyme sites, allowing for easy insertion and manipulation of genes and regulatory elements. This flexibility was crucial for the iterative design and testing phases of pQQC7.
  • Regulatory Elements: The plasmid contains preliminary versions of the promoters, 5' UTRs, and other regulatory sequences that were later fine-tuned in pQQC7. These elements were tested for their expression levels and stability in the chloroplast environment.
  • Selectable Marker and Reporter Gene: pYY11 includes the aadA selectable marker gene and the GFP reporter gene, which were used to test and validate the selection and visualization processes. These genes were driven by early versions of the psbA and Prrn promoters, respectively.
  • Homologous Recombination Sequences: Initial homologous recombination regions (LHRR and RHRR) were included in pYY11 to ensure proper integration into the kiwifruit chloroplast genome. These sequences were later refined and optimized in pQQC7.
  • Stability and Compatibility: pYY11 was designed to be stable and compatible with standard molecular biology techniques, ensuring that it could be efficiently propagated and maintained in bacterial hosts.

Summary:

pYY11 is an intermediate plasmid that played a vital role in the development of the pQQC7 plastid transformation vector. It provided the foundational structure and key components that were further refined and optimized to create the final, highly functional pQQC7 plasmid. This intermediate step was essential for ensuring the robustness and reliability of the final construct, making pYY11 a critical part of our project's success.

Usage:

  • Development and Testing: pYY11 can be used as a reference or starting point for researchers who are interested in the development and optimization of plastid transformation vectors.
  • Educational Tool: It serves as an educational tool to demonstrate the iterative process of plasmid design and the importance of intermediate steps in the development of advanced genetic constructs.

Sequence and Features

BBa_K5044311 SequenceAndFeatures