Difference between revisions of "Part:BBa K5127016:Design"

 
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===Design Notes===
 
===Design Notes===
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The pRed-Aspink expresses the Lambda Red recombinase under the control of an arabinose-inducible promoter. The plasmid also constitutively expresses the pigment protein asPink, which serves as a visual marker to confirm the presence of the plasmid in the host cells (Figure 1).
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The pRed-Aspink expresses the Lambda Red recombinase under the control of an arabinose-inducible promoter. The plasmid also constitutively expresses the chromoprotein, AsPink, which serves as a visual marker to confirm the presence of the plasmid in the host cells (Figure 1).
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<p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/results/21.jpg" width="400" height="auto"/>
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Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.
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Revision as of 01:21, 2 October 2024


Arabinose inducible Lambda Red system with reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1776
    Illegal AgeI site found at 3428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1758


Design Notes

The pRed-Aspink expresses the Lambda Red recombinase under the control of an arabinose-inducible promoter. The plasmid also constitutively expresses the chromoprotein, AsPink, which serves as a visual marker to confirm the presence of the plasmid in the host cells (Figure 1).


Figure 1. Plasmid design of pRed-Aspink. Created by biorender.com.


Source

E. Coli

References