Difference between revisions of "Part:BBa K197028:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K197028 short</partinfo>
 
<partinfo>BBa_K197028 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This is a beta helix
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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+
  
 +
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===
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<pre>
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synthesis
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Construction of Beta Helix Repeats basic part
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PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA (703bp, EcoRI/BamHI)
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Sub into JCA_pBca9495AK-Bca1144      (EcoRI/BamHI, 3171+910, L)
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Product is JCA_pBca9495AK-M10042    {Beta Helix Repeats}
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----------------------------
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Ost005F  Forward oligo for Beta Helix Repeats
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ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg
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Ost006R  Reverse oligo for Beta Helix Repeats
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ctagcGGATCCtgtacgcatgacaaatgctgac
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</pre>
  
 
===References===
 
===References===

Latest revision as of 05:23, 22 October 2009

Beta Helix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Construction of Beta Helix Repeats basic part
PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA (703bp, EcoRI/BamHI)
Sub into JCA_pBca9495AK-Bca1144      (EcoRI/BamHI, 3171+910, L)
Product is JCA_pBca9495AK-M10042     {Beta Helix Repeats}
----------------------------
Ost005F   Forward oligo for Beta Helix Repeats
ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg
Ost006R   Reverse oligo for Beta Helix Repeats
ctagcGGATCCtgtacgcatgacaaatgctgac

References