Difference between revisions of "Part:BBa K197009:Design"

 
(→‎Design Notes)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K197009 short</partinfo>
 
<partinfo>BBa_K197009 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
N/A
+
This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
−
 
+
  
 +
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===
  
−
From genomic source: Eschericia coli.
+
<pre>
 +
PCR ca998/O09ig280 on M10044   (1362, EcoRI/BamHI)
 +
Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L)
 +
Product is pBca9523-B09espP  {<espP(beta)>}
 +
----
 +
ca998 Forward Oligo
 +
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
 +
O09ig280 Reverse Oligo for the removal of the stop codon from {<espP(beta)>}
 +
CGTTAGGATCCgaacgagtaacggaaattagcg
 +
</pre>
  
 
===References===
 
===References===

Latest revision as of 05:17, 22 October 2009

{espP(beta)}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR ca998/O09ig280 on M10044		  	(1362, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09espP   		{<espP(beta)>}
----
ca998	Forward Oligo 
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig280	Reverse Oligo for the removal of the stop codon from {<espP(beta)>}
CGTTAGGATCCgaacgagtaacggaaattagcg

References