Difference between revisions of "Part:BBa K5117021"
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<partinfo>BBa_K5117021 short</partinfo> | <partinfo>BBa_K5117021 short</partinfo> | ||
− | This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>. | + | This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>. |
− | + | ||
P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation. | P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation. | ||
− | + | Information regarding the <i>cotYZ</i> operon can be found in the following databases: | |
− | Information regarding the <i>cotYZ</i> operon can be found | + | |
<html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>, | <html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>, | ||
<html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>. | <html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>. | ||
In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels <i>et al.</i> 2018, unpublished data of Elif Öztel). | In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels <i>et al.</i> 2018, unpublished data of Elif Öztel). | ||
+ | |||
P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page). | P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page). | ||
+ | |||
+ | <b>Biosafety level:</b> S1 | ||
<b>Target organism:</b> <i>Bacillus subtilis</i> | <b>Target organism:</b> <i>Bacillus subtilis</i> | ||
− | <b>Main purpose of use:</b> | + | <b>Main purpose of use:</b> Sporulation-dependent gene expression in the host <i>B. subtilis</i> |
+ | |||
+ | <b> Potential application:</b> Spore surface display | ||
Latest revision as of 23:57, 1 October 2024
PcotYZ
This part contains the regulatory sequence of PcotYZ, which is a promoter of Bacillus subtilis. PcotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. Information regarding the cotYZ operon can be found in the following databases: SubtiWiki, DBTBS.
In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).
PcotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Sporulation-dependent gene expression in the host B. subtilis
Potential application: Spore surface display
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285
Öztel, E. & Mascher T. (2024): unpublished data.