Difference between revisions of "Part:BBa K5117021"

 
 
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<partinfo>BBa_K5117021 short</partinfo>
 
<partinfo>BBa_K5117021 short</partinfo>
  
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The promoter PcotYZ is located upstream the cotYZ operon in the Bacillus subtilis genome.
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This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>.  
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It is active during sporulation and enables sporulation dependent expression of target genes.
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P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation.
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Information regarding the <i>cotYZ</i> operon can be found in the following databases:
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<html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>,
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<html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>.
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In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels <i>et al.</i> 2018, unpublished data of Elif Öztel).
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P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
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<b>Biosafety level:</b> S1
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<b>Target organism:</b> <i>Bacillus subtilis</i>
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<b>Main purpose of use:</b> Sporulation-dependent gene expression in the host <i>B. subtilis</i>
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<b> Potential application:</b> Spore surface display
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117021 SequenceAndFeatures</partinfo>
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===References===
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Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the <i>Bacillus subtilis</i> endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285
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Öztel, E. & Mascher T. (2024): unpublished data.
  
  

Latest revision as of 23:57, 1 October 2024


PcotYZ

This part contains the regulatory sequence of PcotYZ, which is a promoter of Bacillus subtilis. PcotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. Information regarding the cotYZ operon can be found in the following databases: SubtiWiki, DBTBS.

In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).


PcotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Sporulation-dependent gene expression in the host B. subtilis

Potential application: Spore surface display


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285

Öztel, E. & Mascher T. (2024): unpublished data.